The Eskasoni First Nation is a First Nation located in Nova Scotia, Canada.
It administers two reserves that have a combined area of 41.93 km2 (16.19 sq mi):
The Eskasoni First Nation is the most populous First Nation in Nova Scotia and has its own community radio station, CICU-FM, broadcasting at 94.1 MHz.
The Eskasoni First Nation is the home of the Unama'ki Institute of Natural Resources, a Mi'kmaq organization devoted to natural resources and the environment.